Error in re-building vignettes:
  ...
Warning in engine$weave(file, quiet = quiet, encoding = enc) :
  The vignette engine knitr::rmarkdown is not available, because the rmarkdown package is not installed. Please install it.
Loading required package: robustbase
Warning: Removed 29 rows containing missing values (geom_point).
Approximate and calculated Tm varri. This is an expected behaviour 

			 but the calculation should be confirmed with a plot (see examples of diffQ).
15.638859833092533.74599536247882
The distribution of the curve data indicates noise. The data should be visually 
inspected with a plot (see examples of diffQ).
Approximate and calculated Tm varri. This is an expected behaviour 

			 but the calculation should be confirmed with a plot (see examples of diffQ).
12.139270996656312.307692307692310.3617975173708
The distribution of the curve data indicates noise. The data should be visually 
inspected with a plot (see examples of diffQ).
The distribution of the curve data indicates noise. The data should be visually 
inspected with a plot (see examples of diffQ).
Approximate and calculated Tm varri. This is an expected behaviour 

			 but the calculation should be confirmed with a plot (see examples of diffQ).
16.42065372042111612.6709691596414
The distribution of the curve data indicates noise. The data should be visually 
inspected with a plot (see examples of diffQ).
Approximate and calculated Tm varri. This is an expected behaviour 

			 but the calculation should be confirmed with a plot (see examples of diffQ).
6.533866827983022021.9375753397427
The Tm calculation (fit, adj. R squared ~ 0.849, NRMSE ~ 0.088) is not optimal presumably
due to noisy data. Check raw melting curve (see examples of diffQ).
knitr hook "anchor" is now available
Approximate and calculated Tm varri. This is an expected behaviour 

			 but the calculation should be confirmed with a plot (see examples of diffQ).
15.638859833092533.74599536247882
The distribution of the curve data indicates noise. The data should be visually 
inspected with a plot (see examples of diffQ).
Approximate and calculated Tm varri. This is an expected behaviour 

			 but the calculation should be confirmed with a plot (see examples of diffQ).
12.139270996656312.307692307692310.3617975173708
The distribution of the curve data indicates noise. The data should be visually 
inspected with a plot (see examples of diffQ).
The distribution of the curve data indicates noise. The data should be visually 
inspected with a plot (see examples of diffQ).
Approximate and calculated Tm varri. This is an expected behaviour 

			 but the calculation should be confirmed with a plot (see examples of diffQ).
16.42065372042111612.6709691596414
The distribution of the curve data indicates noise. The data should be visually 
inspected with a plot (see examples of diffQ).
Approximate and calculated Tm varri. This is an expected behaviour 

			 but the calculation should be confirmed with a plot (see examples of diffQ).
6.533866827983022021.9375753397427
The Tm calculation (fit, adj. R squared ~ 0.849, NRMSE ~ 0.088) is not optimal presumably
due to noisy data. Check raw melting curve (see examples of diffQ).
Quitting from lines 127-158 (usingRDML_with_other_R_packages.Rmd) 
Error: processing vignette ‘usingRDML_with_other_R_packages.Rmd’ failed with diagnostics:
there is no package called 'qpcR'
Execution halted