• using R version 4.5.2 Patched (2026-01-31 r89382)
  • using platform: aarch64-apple-darwin20
  • R was compiled by     Apple clang version 16.0.0 (clang-1600.0.26.6)     GNU Fortran (GCC) 14.2.0
  • running under: macOS Ventura 13.7.8
  • using session charset: UTF-8
  • checking for file ‘ggpicrust2/DESCRIPTION’ ... OK
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  • this is package ‘ggpicrust2’ version ‘2.5.10’
  • package encoding: UTF-8
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  • checking package dependencies ... INFO Packages suggested but not available for checking:   'ALDEx2', 'metagenomeSeq', 'lefser'
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  • checking whether package ‘ggpicrust2’ can be installed ... [3s/3s] OK See the install log for details.
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  • checking for unstated dependencies in ‘tests’ ... OK
  • checking tests ... [8s/9s] ERROR   Running ‘testthat.R’ [8s/9s] Running the tests in ‘tests/testthat.R’ failed. Complete output:   > library(testthat)   > library(ggpicrust2)   Loading required package: ggpicrust2   To cite ggpicrust2 in publications use:   Chen Yang, Jiahao Mai, Xuan Cao, Aaron Burberry, Fabio Cominelli, Liangliang Zhang, ggpicrust2: an R package for PICRUSt2 predicted functional profile analysis and visualization, Bioinformatics, Volume 39, Issue 8, August 2023, btad470, https://doi.org/10.1093/bioinformatics/btad470   >   > test_check("ggpicrust2")   Reading input data...      Performing pathway differential abundance analysis...      Annotating pathways...      Creating pathway error bar plots...      Plot 1 skipped (no data for method: mock_method)      ggpicrust2 analysis completed.      Note: Preranked GSEA methods (fgsea, clusterProfiler) do not account for inter-gene correlations, which may lead to unreliable p-values (Wu et al., 2012). Consider using method='camera' or method='fry' for more reliable statistical inference.   Using column 'sample_name' as sample identifier        |     | | 0%     |     | | 1%     |     |= | 1%     |     |= | 2%     |     |== | 2%     |     |== | 3%     |     |== | 4%     |     |=== | 4%     |     |=== | 5%     |     |==== | 5%     |     |==== | 6%     |     |===== | 7%     |     |===== | 8%     |     |====== | 8%     |     |====== | 9%     |     |======= | 9%     |     |======= | 10%     |     |======= | 11%     |     |======== | 11%     |     |======== | 12%     |     |========= | 12%     |     |========= | 13%     |     |========== | 14%     |     |========== | 15%     |     |=========== | 15%     |     |=========== | 16%     |     |============ | 17%     |     |============ | 18%     |     |============= | 18%     |     |============= | 19%     |     |============== | 19%     |     |============== | 20%     |     |============== | 21%     |     |=============== | 21%     | 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_problems/test-pathway_daa-150.R   Saving _problems/test-pathway_daa-181.R   Saving _problems/test-pathway_daa-221.R   Saving _problems/test-pathway_daa-233.R   Excluded 1 pathways with missing annotations. Use 'pathway_annotation' to add them.   Excluded 1 rows with missing 'pathway_name' annotations.   Saving _problems/test-pathway_errorbar-232.R   Note: Preranked GSEA methods (fgsea, clusterProfiler) do not account for inter-gene correlations, which may lead to unreliable p-values (Wu et al., 2012). Consider using method='camera' or method='fry' for more reliable statistical inference.   Using column 'sample_name' as sample identifier   Using column 'sample_name' as sample identifier   Testing 2 gene sets (filtered from 2 by size constraints)   Using column 'sample' as sample identifier   The Sample Names in order from left to right are:   S1, S2, S3, S4   The Group Levels in order from left to right are:   A, A, B, B   Using column 'sample' as sample identifier   The Sample Names in order from left to right are:   S1, S2, S3, S4   The Group Levels in order from left to right are:   A, A, B, B   Using column 'sample' as sample identifier   The Sample Names in order from left to right are:   S1, S2, S3, S4   The Group Levels in order from left to right are:   A, A, B, B   Using column 'sample_name' as sample identifier   Using column 'sample_name' as sample identifier   Using column 'sample_name' as sample identifier   Too few points to calculate an ellipse   Too few points to calculate an ellipse   Using column 'sample_name' as sample identifier   Using column 'sample_name' as sample identifier   Using column 'sample_name' as sample identifier   Using column 'sample_name' as sample identifier        |     | | 0%     |     | | 1%     |     |= | 1%     |     |= | 2%     |     |== | 2%     |     |== | 3%     |     |== | 4%     |     |=== | 4%     |     |=== | 5%     |     |==== | 5%     |     |==== | 6%     |     |===== | 7%     |     |===== | 8%     |     |====== | 8%     |     |====== | 9%     |     |======= | 9%     |     |======= | 10%     |     |======= | 11%     |     |======== | 11%     |     |======== | 12%     |     |========= | 12%     |     |========= | 13%     |     |========== | 14%     |     |========== | 15%     |     |=========== | 15%     |     |=========== | 16%     |     |============ | 17%     |     |============ | 18%     |     |============= | 18%     |     |============= | 19%     |     |============== | 19%     |     |============== | 20%     |     |============== | 21%     |     |=============== | 21%     |     |=============== | 22%     |     |================ | 22%     |     |================ | 23%     |     |================= | 24%     |     |================= | 25%     |     |================== | 25%     |     |================== | 26%     |     |=================== | 26%     |     |=================== | 27%     |     |=================== | 28%     |     |==================== | 28%     |     |==================== | 29%     |     |===================== | 29%     |     |===================== | 30%     |     |===================== | 31%     |     |====================== | 31%     |     |====================== | 32%     |     |======================= | 32%     |     |======================= | 33%     |     |======================= | 34%     |     |======================== | 34%     |     |======================== | 35%     |     |========================= | 35%     |     |========================= | 36%     | 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| 7%     |     |===== | 8%     |     |====== | 8%     |     |====== | 9%     |     |======= | 9%     |     |======= | 10%     |     |======= | 11%     |     |======== | 11%     |     |======== | 12%     |     |========= | 12%     |     |========= | 13%     |     |========== | 14%     |     |========== | 15%     |     |=========== | 15%     |     |=========== | 16%     |     |============ | 17%     |     |============ | 18%     |     |============= | 18%     |     |============= | 19%     |     |============== | 19%     |     |============== | 20%     |     |============== | 21%     |     |=============== | 21%     |     |=============== | 22%     |     |================ | 22%     |     |================ | 23%     |     |================= | 24%     |     |================= | 25%     |     |================== | 25%     |     |================== | 26%     |     |=================== | 26%     |     |=================== | 27%     |     |=================== | 28%     | 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    |     |===================================================================== | 98%     |     |===================================================================== | 99%     |     |======================================================================| 99%     |     |======================================================================| 100%        |     | | 0%     |     | | 1%     |     |= | 1%     |     |= | 2%     |     |== | 2%     |     |== | 3%     |     |== | 4%     |     |=== | 4%     |     |=== | 5%     |     |==== | 5%     |     |==== | 6%     |     |===== | 7%     |     |===== | 8%     |     |====== | 8%     |     |====== | 9%     |     |======= | 9%     |     |======= | 10%     |     |======= | 11%     |     |======== | 11%     |     |======== | 12%     |     |========= | 12%     |     |========= | 13%     |     |========== | 14%     |     |========== | 15%     |     |=========== | 15%     |     |=========== | 16%     |     |============ | 17%     |     |============ | 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| 4%     |     |=== | 5%     |     |==== | 5%     |     |==== | 6%     |     |===== | 7%     |     |===== | 8%     |     |====== | 8%     |     |====== | 9%     |     |======= | 9%     |     |======= | 10%     |     |======= | 11%     |     |======== | 11%     |     |======== | 12%     |     |========= | 12%     |     |========= | 13%     |     |========== | 14%     |     |========== | 15%     |     |=========== | 15%     |     |=========== | 16%     |     |============ | 17%     |     |============ | 18%     |     |============= | 18%     |     |============= | 19%     |     |============== | 19%     |     |============== | 20%     |     |============== | 21%     |     |=============== | 21%     |     |=============== | 22%     |     |================ | 22%     |     |================ | 23%     |     |================= | 24%     |     |================= | 25%     |     |================== | 25%     |     |================== | 26%     |     |=================== | 26%     | 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(4): 'test-pathway_ridgeplot.R:25:3',     'test-pathway_ridgeplot.R:42:3', 'test-pathway_ridgeplot.R:65:3',     'test-pathway_volcano.R:82:3'   • Set GGPICRUST2_RUN_E2E_TESTS=true to run full ggpicrust2 end-to-end tests.     (1): 'test-ggpicrust2-return-structure.R:4:3'   • Set GGPICRUST2_RUN_EXTENDED_DAA_TESTS=true to run extended DAA method tests.     (1): 'test-pathway_daa.R:102:3'   • Set GGPICRUST2_RUN_NETWORK_TESTS=true to run network-dependent KEGG tests.     (2): 'test-pathway_annotation.R:36:3', 'test-pathway_annotation.R:55:3'   • {lefser} is not installed (1): 'test-pathway_daa.R:293:3'      ══ Failed tests ════════════════════════════════════════════════════════════════   ── Error ('test-pathway_daa.R:32:3'): pathway_daa works with basic inputs ──────   Error in `require_package(method_packages[[daa_method]], purpose = daa_method)`: Package 'ALDEx2' is required for ALDEx2. Install with: BiocManager::install('ALDEx2')   Backtrace:       ▆    1. └─ggpicrust2::pathway_daa(td$abundance, td$metadata, "group", daa_method = "ALDEx2") at test-pathway_daa.R:32:3    2. └─ggpicrust2:::require_package(method_packages[[daa_method]], purpose = daa_method)   ── Error ('test-pathway_daa.R:49:3'): pathway_daa validates inputs correctly ───   Error in `require_package(method_packages[[daa_method]], purpose = daa_method)`: Package 'ALDEx2' is required for ALDEx2. Install with: BiocManager::install('ALDEx2')   Backtrace:       ▆    1. ├─testthat::expect_error(...) at test-pathway_daa.R:49:3    2. │ └─testthat:::expect_condition_matching_(...)    3. │ └─testthat:::quasi_capture(...)    4. │ ├─testthat (local) .capture(...)    5. │ │ └─base::withCallingHandlers(...)    6. │ └─rlang::eval_bare(quo_get_expr(.quo), quo_get_env(.quo))    7. └─ggpicrust2::pathway_daa(...)    8. └─ggpicrust2:::require_package(method_packages[[daa_method]], purpose = daa_method)   ── Error ('test-pathway_daa.R:86:5'): pathway_daa core methods produce expected results ──   Error in `require_package(method_packages[[daa_method]], purpose = daa_method)`: Package 'ALDEx2' is required for ALDEx2. Install with: BiocManager::install('ALDEx2')   Backtrace:       ▆    1. ├─base::suppressWarnings(...) at test-pathway_daa.R:86:5    2. │ └─base::withCallingHandlers(...)    3. └─ggpicrust2::pathway_daa(abundance, metadata, "group", daa_method = method)    4. └─ggpicrust2:::require_package(method_packages[[daa_method]], purpose = daa_method)   ── Error ('test-pathway_daa.R:149:3'): pathway_daa handles sample selection correctly ──   Error in `require_package(method_packages[[daa_method]], purpose = daa_method)`: Package 'ALDEx2' is required for ALDEx2. Install with: BiocManager::install('ALDEx2')   Backtrace:       ▆    1. └─ggpicrust2::pathway_daa(...) at test-pathway_daa.R:149:3    2. └─ggpicrust2:::require_package(method_packages[[daa_method]], purpose = daa_method)   ── Error ('test-pathway_daa.R:180:3'): pathway_daa handles factor levels correctly with subset ──   Error in `require_package(method_packages[[daa_method]], purpose = daa_method)`: Package 'ALDEx2' is required for ALDEx2. Install with: BiocManager::install('ALDEx2')   Backtrace:       ▆    1. └─ggpicrust2::pathway_daa(...) at test-pathway_daa.R:180:3    2. └─ggpicrust2:::require_package(method_packages[[daa_method]], purpose = daa_method)   ── Error ('test-pathway_daa.R:220:3'): pathway_daa handles p-value adjustment correctly ──   Error in `require_package(method_packages[[daa_method]], purpose = daa_method)`: Package 'ALDEx2' is required for ALDEx2. Install with: BiocManager::install('ALDEx2')   Backtrace:       ▆    1. └─ggpicrust2::pathway_daa(...) at test-pathway_daa.R:220:3    2. └─ggpicrust2:::require_package(method_packages[[daa_method]], purpose = daa_method)   ── Error ('test-pathway_daa.R:232:3'): pathway_daa include_abundance_stats parameter works correctly ──   Error in `require_package(method_packages[[daa_method]], purpose = daa_method)`: Package 'ALDEx2' is required for ALDEx2. Install with: BiocManager::install('ALDEx2')   Backtrace:       ▆    1. └─ggpicrust2::pathway_daa(...) at test-pathway_daa.R:232:3    2. └─ggpicrust2:::require_package(method_packages[[daa_method]], purpose = daa_method)   ── Error ('test-pathway_errorbar.R:227:3'): pathway_errorbar_table function works correctly ──   Error in `require_package(method_packages[[daa_method]], purpose = daa_method)`: Package 'ALDEx2' is required for ALDEx2. Install with: BiocManager::install('ALDEx2')   Backtrace:       ▆    1. └─ggpicrust2::pathway_daa(...) at test-pathway_errorbar.R:227:3    2. └─ggpicrust2:::require_package(method_packages[[daa_method]], purpose = daa_method)      [ FAIL 8 | WARN 2 | SKIP 9 | PASS 151 ]   Error:   ! Test failures.   Execution halted
  • checking for unstated dependencies in vignettes ... OK
  • checking package vignettes ... OK
  • checking re-building of vignette outputs ... [3s/4s] OK
  • checking PDF version of manual ... [2s/2s] OK
  • DONE Status: 1 ERROR
  • using check arguments '--no-clean-on-error '